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In contrast to random primers, oligo (dT) primers are not suitable for degraded samples but are outstanding for setting up cDNA libraries and advisable to be used with reverse transcriptases (RTs) as Superscript IV RT.
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Enable This controls if the primer must span an exon junction in your mRNA template. The choice "Primer need to span an exon-exon junction" will direct This system to return a minimum of 1 primer (within a supplied primer pair) that spans an exon-exon junction.
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